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To check whether a request has been made is from Ajax or not you can use $_SERVER[HTTP_X_REQUESTED_WITH]. See the following example:

<?php
if(strtolower($_SERVER[HTTP_X_REQUESTED_WITH])==xmlhttprequest){
 echo "The request is from an Ajax Call";
}else{
 echo "The request is not from an Ajax Call";
}
?>

There you go. You now know how to check whether request made to file is from an Ajax Call or not in PHP.